Lawson China (LAWSON) sample liquid nitrogen grinder, DHFSTPRP-LN24/48 is specially used for grinding heat sensitive substances, various animal and plant tissues, microorganisms, rubber, plastics, food, medicine, coal, oil shale, wax products, PE, PS, textiles, resins, etc., and materials that are tough at room temperature and difficult to pulverize.
The liquid nitrogen mill first freezes the above substances below the embrittlement point, and then grinds them to the desired fineness in the grinder, and the original components are not destroyed. And the flammable and explosive substances at normal temperature can also be pulverized at a low temperature, and the effect is more remarkable.
This instrument is also known as multi-sample tissue grinding machine, rapid tissue grinding machine, multi-sample tissue homogenizer, and rapid sample homogenization system.
Steps:
1. Manual operation
(1) Manually immerse the adapter and grinding jar in a liquid nitrogen sample and quickly cool to -196 degrees. Prevent sample degradation or allow changes in the physical properties of the sample.
(2) Attach the adapter and the grinding jar to the grinder. Cover the safety cover and lock the safety lock to operate.
2. Automatic operation
(1) Attach the adapter and the grinding jar to the grinder. Cover the safety cover and lock the safety lock to operate.
(2) Open the liquid nitrogen connection device, start liquid nitrogen delivery, or turn on the air refrigerator (to achieve 0 degree cooling).
(3) Turn on the liquid nitrogen control switch. After the liquid nitrogen flows into the crushing tank, it will be physically cooled. After a minute or so, quickly cool down to -192 degrees.
(4) Turn on the grinding button to operate. In just 15 seconds, the object smashes instantly.
(5) The liquid nitrogen flow rate can be controlled at all times, and can be filled at any time during the operation.
Features :
A. High accuracy and reproducibility of data
1. The sample is always at liquid nitrogen temperature during the grinding process.
2. The sample is cooled to -100 ° C in 1 minute and -196 ° C in 4 minutes and 30 seconds.
3. The vial is fully enclosed during the crushing process and the sample is completely retained in the bottle to ensure control of dangerous and important samples.
4. The sample bottles are sealed independently, and it is very convenient to clean after each crushing to avoid cross-contamination and external pollution between samples.
B. Versatility, user-friendly design
1. Touch display, which can set parameters such as grinding time, cycle time and grinding operation frequency (optional).
2. Movable control panel (optional).
3. Can store 10 programs (optional).
4. Can be sampled by 1.51.5 2ML 5ML 10ML 25ML 50ML and other sample tubes.
C. Durability
1. Only one moving part, the collider, greatly reduces mechanical stress and extends equipment life.
2. Strong technical support: Ningbo Luoshang Company has more than 500 users, professional sample pretreatment equipment production.
Grindable samples:
1. Plant tissue: roots, stems, leaves, flowers, fruits, seeds, etc.
2. Animal tissue: brain, heart, lung, stomach, liver, thymus, kidney, intestine, lymph nodes, muscles, bones, etc.
3. Fungal bacteria: yeast, E. coli, etc.
4. Food and Drugs: All kinds of food, pills, etc.
5. Volatile samples: coal, oil shale, wax products, etc.
6. Plastics, polymers: PE, PS, textiles, resins, etc.
DHFSTPRP-LN, the sample liquid nitrogen grinder adopts a special vertical vertical vibration mode, which is used for high-frequency reciprocating vibration, impact and shear of grinding beads (zirconia, steel balls, glass beads, ceramic beads). Fast implementation goals. The ground sample is made more adequate, more uniform, sample repeatability, and no cross-contamination between samples.
A large number of operations and good results. Efficient and fast work can complete the grinding of 2×24 and 2×48 samples in 1 minute. Save time and effort, between batches, the difference within the batch is small. The extracted protein is more active and the nucleic acid fragment is longer.
No cross-contamination The sample tube is fully enclosed during the crushing process and disposable centrifuge tubes and beads can be used. The sample is completely retained in the tube to avoid cross-contamination between samples and external contamination.
Easy to operate 1. Built-in program controller, which can set parameters such as grinding time and vibration frequency of the rotor;
2. User-friendly operation interface.
Good stability 1. Vertical oscillating mode, more complete grinding and better stability;
2. During the operation of the instrument, the noise is less than 55dB and will not cause interference to other experiments or instruments.
Convenient for low temperature operation When a low temperature grinding environment is required, the adapter with the sample can be immersed in liquid nitrogen for 1-2 minutes, removed and transferred to the main unit for quick fixing to start grinding. It does not need to be re-frozen and save liquid nitrogen.
Good repeatability The same procedure was set for the same tissue sample to achieve the same grinding effect. The working time is short and the sample temperature does not rise.
1) Has five systems, sample grinding system, liquid nitrogen storage system, liquid nitrogen control system, circuit control system, insulation and cold preservation system.
2) Applications: Biology, Food, Agriculture, Pharmaceuticals, Chemistry / Synthetic Materials, Engineering / Electronics, Building Materials, Glass / Ceramics.
3) Automatic filling of liquid nitrogen: The liquid nitrogen flow can be controlled at all times, and can be filled at any time during the operation.
4) There are two ways to grind liquid nitrogen: manual and fully automatic.
5) Have a liquid nitrogen connection device that is automatically transported from the storage system to the sample grinding system.
6) Low temperature or liquid nitrogen grinding is possible: Yes, it can be connected to a liquid nitrogen tank (type II) or an air cooler (type I).
7) The outer casing is made of hard steel, which avoids the plastic cover and affects safety.
8) Sample characteristics: hard, medium hard, soft, brittle, elastic, fiber-containing.
9) Principle of crushing: low temperature freezing force, mechanical impact force, physical friction.
10) Temperature range: -196°C-100°C
11) Injection size: ≤ 8 mm
12) Discharge size: 0~ 5 μm
13) Standard machine processing sample quantity: 50ml*2 (can be customized)
14) Grinding tank size: 1.5ml/2ml/5 ml /10mL / 25 ml / 35 ml / 50 ml. Can be customized.
15) Grinding cans can be placed at one time without repeated resettlement, prolonging working time and affecting sample temperature.
16) Grinding speed: 1-70HZ adjustable (30-2100 rpm / 100-7000RPM).
17) Anti-vibration principle: DHFSTPRP-1 innovative shock-proof principle, and special three-dimensional vibration mode of up and down and left and right shaking. The sample is in figure-shaped three-dimensional motion in space, which can be applied to cracking various animal and plant tissues and microorganisms, which is convenient and quick to extract. DNA, RNA, protein.
18) Typical liquid nitrogen pulverizer: 15 seconds
19) Grinding tank material: stainless steel, polytetrafluorotetrafluoroethylene (Teflon)
20) Grinding ball material: alloy steel, chrome steel, zirconia, tungsten carbide, quartz sand
21) Acceleration: reach a large speed in 2 seconds
22) Slow down: reach lower speed in 2 seconds
Host one DHFSTPRP-LN24/48
Automatic liquid nitrogen system
Adapter Ø2ml set
Refrigeration adapter Ø2ml set or Ø1.5ml set (2 choice 1)
Grinding beads 1.Ø5 imported stainless steel grinding beads 1000 or Ø5 imported ceramic grinding beads 1000 (2 choice 1);
2.Ø3 imported stainless steel grinding beads 1000 pieces.
Special grinding tube 200
Special bead gun set (optional)
Extracted Object: Animal Tissue - DNA LW-D-P1 (optional)
Extraction object: animal tissue-RNA LW-D-P2 (optional)
Extracted Object: Animal Tissue - Protein LW-D-P3 (optional)
Extraction object: plant tissue - DNA LW-Z-P1 (optional)
Extraction object: plant tissue-RNA LW-Z-P2 (optional)
Extraction object: plant tissue - protein LW-Z-P3 (optional)
Extraction target: Microbial-DNA LW-W-P1 (optional)
Extraction target: Microbial-RNA LW-W-P2 (optional)
Extraction object: microbial-protein LW-W-P3 (optional)
* The selected LAWSON kit can be used to extract DNA/RNA protein in samples quickly, efficiently, stably and in batches.
* 1HZ = 30 rev / sec 1HZ = 100RPM
* Regular adapters can be placed in the refrigerator to give the sample a cold environment while working. It can be equipped with an adapter with refrigeration and can be immersed in liquid nitrogen for more than 30 minutes.
* The noise emission value for the working environment depends on the type of sample and the setting of the grinder. The parameters in the table are empty
* A small number of samples commonly used for DNA and RNA isolation can be prepared in disposable centrifuge tubes. Adapters made of Teflon can be used to hold 5 or 10 disposable sample tubes for cell disruption. The cell tissue in the mill is quickly and efficiently broken in a very short time, eliminating the need for additional cryogenic refrigeration.
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